Recombinant DNA Technology versus PCR

Recombinant DNA:

-Cloning and expression of definite gene or DNA segment is done.

-In vivo method

-Vector needed

-The main enzyme is restriction endonuclease and DNA ligase

-In genetic engineering, DNA plays an important role

Polymerase Chain Reaction (PCR)

-Only cloning of a definite gene or DNA segment is done.

-In vitro method (Outside of living cell)

-Vector not needed

-The main enzyme is heat stable DNA polymerase

-Least importance in genetic engineering.