Recombinant DNA Technology versus PCR
Recombinant DNA:
-Cloning and expression of definite gene or DNA segment is done.
-In vivo method
-Vector needed
-The main enzyme is restriction endonuclease and DNA ligase
-In genetic engineering, DNA plays an important role
Polymerase Chain Reaction (PCR)
-Only cloning of a definite gene or DNA segment is done.
-In vitro method (Outside of living cell)
-Vector not needed
-The main enzyme is heat stable DNA polymerase
-Least importance in genetic engineering.